We propose to identify genes in bone cells that respond specifically to the localized production of human growth hormone (hGH) in marrow. The differential display polymerase chain reaction (DD-PCR) technique will be employed to select and clone cDNAs that are activated or repressed in HS2+BetachGH transgenic mouse bones compared to normal bones. The bones of the transgenic mice have more density, increased cortical thickness, and enhanced biomechanical qualities compared to their controls. Our approach will identify genes that experience major changes in their expression as a consequence of transgene expression: genes that are already known or predicted, as well as those that have not previously been characterized or associated with osteogenesis. The particular relevance of each gene to alveolar bone deposition will be confirmed. Identification of the genes acting downstream of the growth hormone stimulation is critical to understanding the mechanism of the effect and ultimately transferring this technology to clinical applications.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Small Research Grants (R03)
Project #
7R03DE012054-03
Application #
6024279
Study Section
NIDCR Special Grants Review Committee (DSR)
Project Start
1997-08-01
Project End
2001-07-31
Budget Start
1999-02-15
Budget End
2001-07-31
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Northeast Ohio Medical University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
City
Rootstown
State
OH
Country
United States
Zip Code
44272