The chemotactic sensory transducing receptor proteins of Eschericia Coli represent an extremely attractive system for X- ray crystallographic structural studies. These proteins (60,000 kilodaltons) span the inner membrane. In the periplasmic space, an N-terminal domain binds ligands, causing information to be transmitted across the membrane and transduced into a form which can modify the swimming behavior of these bacteria. In order to better understand how receptors interact with ligands, how information is transmitted across biological membranes and how signals are generated, crystallization of wild type and mutant forms of these molecules followed by X-ray structural studies are proposed. Preliminary results indicate that these molecules may crystallize in forms useful for structural studies. Crystalline rosettes of a large fragment of one of the E. Coli receptor/transducers have already been obtained from detergent-containing solutions using standard crystallization procedures.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Small Research Grants (R03)
Project #
5R03DK039848-02
Application #
3426134
Study Section
Diabetes and Digestive and Kidney Diseases Special Grants Review Committee (DDK)
Project Start
1987-09-01
Project End
1989-08-31
Budget Start
1988-09-01
Budget End
1989-08-31
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Oregon
Department
Type
Schools of Arts and Sciences
DUNS #
948117312
City
Eugene
State
OR
Country
United States
Zip Code
97403