The transcription factor, NFkB, is a critical factor in the cellular response to various types of stress. NFkB activation is associated with cell proliferation and occurs in many tumors. The investigators have shown that peroxisome proliferators, which cause oxidative stress and act as hepatic carcinogens in rodents, can activate NFkB in the liver. This activation can be blocked by antioxidants. NFkB activation is mediated by a signaling pathway that leads to the phosphorylation and subsequent polyubiquitination and degradation of IkB. Recently, several groups have used in vitro systems to show that the SUMO can be conjugated to IkBa in a manner that is similar to polyubiquitination of this molecule. These studies raise the interesting possibility that SUMO-modified forms of IkBa may be involved in the cellular response to stress. The investigators have identified a novel form of IkBa that is found in the livers of mice that have been treated with peroxisome proliferators. This modification is consistent with the possibility that this is a SUMO-modified form of IkBa. They hypothesize that this novel form of IkBa may be an important regulator of NFkB signaling in the livers of mice treated with peroxisome proliferators. In this grant, they propose to characterize the slower migrating form of IkBa in the livers of mice treated with peroxisome proliferators and determine whether this indeed is due to SUMO-modification. In addition, since this novel IkBa form is induced by long-term treatment with peroxisome proliferators, which are known to cause oxidative stress, the investigators will determine whether this novel IkBa can be modulated by dietary antioxidants. Overall, these studies may help elucidate additional mechanisms that control NFkB activity in organisms.
| Glauert, Howard P; Eyigor, Aysegul; Tharappel, Job C et al. (2006) Inhibition of hepatocarcinogenesis by the deletion of the p50 subunit of NF-kappaB in mice administered the peroxisome proliferator Wy-14,643. Toxicol Sci 90:331-6 |
