description): Homeobox (Hox) genes are essential determinants of axial patterning during embryonic development of a wide variety of organisms ranging from the fruitfly to man. In all species studied to date, the Hox genes are closely linked on the chromosome in clusters of about 5-11 genes. Once genes in the clusters are expressed, their expression pattern is maintained by the activity of the Polycomb (PcG) and trithorax (trxG) group proteins. PcG proteins maintain Hox genes in an off state, while trxG proteins maintain expression in an on state through multiple cell divisions. This proposal aims to identify mechanisms involved in this epigenetic control of Hox gene expression by PcG and trxG proteins. Both protein groups presumably work by affecting accessibility of the chromatin to transcription factors by an unknown mechanism. This study aims to first, determine the histone acetylation state of the mammalian clusters. Second, the investigators will map the interaction of PcG and trxG proteins across the Hoxb cluster and correlate these interactions with histone acetylation. As a model system, the investigators will use the embryonal carcinoma cell line, P19, which can be induced with retinoic acid to activate Hox gene expression leading to neuronal cell types.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Small Research Grants (R03)
Project #
1R03HD039427-01
Application #
6191163
Study Section
Pediatrics Subcommittee (CHHD)
Program Officer
Moody, Sally Ann
Project Start
2000-07-10
Project End
2002-06-30
Budget Start
2000-07-10
Budget End
2001-06-30
Support Year
1
Fiscal Year
2000
Total Cost
$76,500
Indirect Cost
Name
University of Cincinnati
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
041064767
City
Cincinnati
State
OH
Country
United States
Zip Code
45221