Abstract

Application) A central question in developmental biology is how a multicellular organism can arise from a single cell. In an effort to understand the complexity of the restricted expression of specific subsets of genes to a particular cell or tissue type, the investigators have compared the transcriptomes (the set of expressed genes) and propose to initiate preliminary studies to determine the feasibility of comparing the proteomes (the set of proteins) of immature and mature chicken dorsal root ganglia (DRG). The objective is to determine the function of a differentially expressed molecule, neural epidermal growth-factor like (NEL), and to extend the analysis of molecular differences to the proteome level.
Specific Aim 1 proposes to determine the function of NEL during sensory neurogenesis.
Specific Aim 2 plans to determine the molecular differences in the proteomes between immature and mature DRG. A proteomic analysis will be initiated by running 2D gels on E4.5 and E8.5 DRG and, based on their resolution, the feasibility of this approach will be determined. If successful, the investigators can conduct protein identification through mass spectrometric analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Small Research Grants (R03)
Project #
5R03HD040343-02
Application #
6530560
Study Section
Pediatrics Subcommittee (CHHD)
Program Officer
Henken, Deborah B
Project Start
2001-03-14
Project End
2003-02-28
Budget Start
2002-03-01
Budget End
2003-02-28
Support Year
2
Fiscal Year
2002
Total Cost
$70,000
Indirect Cost

  Institution

Name
Montana State University Bozeman
Department
Anatomy/Cell Biology
Type
Schools of Arts and Sciences
DUNS #
City
Bozeman
State
MT
Country
United States
Zip Code
59717

  Publications

Nielsen, Katherine M; Chaverra, Martha; Hapner, Sharon J et al. (2004) PACAP promotes sensory neuron differentiation: blockade by neurotrophic factors. Mol Cell Neurosci 25:629-41
Nelson, Branden R; Claes, Karen; Todd, Valerie et al. (2004) NELL2 promotes motor and sensory neuron differentiation and stimulates mitogenesis in DRG in vivo. Dev Biol 270:322-35
Nelson, Branden R; Sadhu, Meru; Kasemeier, Jennifer C et al. (2004) Identification of genes regulating sensory neuron genesis and differentiation in the avian dorsal root ganglia. Dev Dyn 229:618-29
Nelson, Branden R; Matsuhashi, Sachiko; Lefcort, Frances (2002) Restricted neural epidermal growth factor-like like 2 (NELL2) expression during muscle and neuronal differentiation. Mech Dev 119 Suppl 1:S11-9
Nelson, Branden R; Matsuhashi, Sachiko; Lefcort, Frances (2002) Restricted neural epidermal growth factor-like like 2 (NELL2) expression during muscle and neuronal differentiation. Gene Expr Patterns 2:7-15