The purpose of this application for grant support is to allow a collaborative study on the topography of glycosylation reactions in the rough endoplasmic reticulum between Dr. Carlos Hirschberg's laboratory (University of Massachusetts Medical Center, Worcester, MA) and Dr. Armando Parodi's laboratory (Fundacion Compomar, Buenos Aires, Argentina). The long term goals of this proposal are to understand how precursors in protein and lipid glycosylation can be translocated across the membrane of the endoplasmic reticulum, from the cytosolic side to the lumenal side. This translocation is believed to play an important regulatory role in protein and lipid glycosylation.
The Specific Aims of this proposal are to determine whether dolichol-P- glucose is synthesized on the cytosolic side of the endoplasmic reticulum membrane and if so, by what mechanism this lipid-saccharide is subsequently translocated across the membrane into the lumen where it serves as a substrate for further glycosylation reactions. These studies will complement ongoing research in Dr. Hirschberg's laboratory dealing with the cell biology, biochemistry & molecular biology of glycosylation reactions, with Dr. Parodi's laboratory on the chemistry and biochemistry of dolichol oligosaccharides.

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Small Research Grants (R03)
Project #
5R03TW000027-02
Application #
2291423
Study Section
Special Emphasis Panel (SRC)
Project Start
1992-04-01
Project End
1995-09-30
Budget Start
1993-10-01
Budget End
1994-09-30
Support Year
2
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Biochemistry
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code
01655