Abstract

This is a renewal proposal for a comparative study of cytoplasmic and organelle seryl tRNA synthetases (SerRS) from yeast and plants, Arabidopsis and maize.
Specific aims i nclude the cloning, sequencing and expression of the mitochondrial seryl tRNA synthetase from S. cerevisiae, the chloroplast SerRS from maize and the cytoplasmic SerRS from Arabidopsis. Substrate binding domains of the various isozymes will be analyzed by site-directed mutagenesis. An additional goal is the identification of the nucleotides in seryl-tRNA's which are responsible for recognition by their cognate synthetases.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Small Research Grants (R03)
Project #
5R03TW000166-06
Application #
6056770
Study Section
International and Cooperative Projects 1 Study Section (ICP)
Program Officer
Michels, Kathleen M
Project Start
1993-07-01
Project End
2001-08-31
Budget Start
1999-09-01
Budget End
2001-08-31
Support Year
6
Fiscal Year
2000
Total Cost
$25,200
Indirect Cost

  Institution

Name
Yale University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520