The binding of macromolecules (e.g. antibodies) and macromolecular assemblies (e.g. viruses) to cell surfaces is critical to a host of biologically and medically important processes. The current research proposal attempts to disclose the mechanistic details of such binding via the study of simple and controllable models involving the interaction of amphiphilic polymers with vesicular systems. The polymers are both negatively and positively charged, and they differ in their charge density and degree of polymerization. The vesicles with which they interact differ in curvature (small, large, and giant) and in their content of charged lipid. Association of the polymers with the vesicles will induce structural rearrangement, including lateral lipid segregation (""""""""domains""""""""), pore formation, and flip-flop all of which will be examined as a function of polymer and vesicle composition. Polymer-vesicle interaction has also been shown to affect transport of an antitumor drug, doxorubicin, an effect that will be further studied owing to its importance in the drug delivery arena. Finally, complexation of the polymers with DNA will be examined with respect to the association with, and transport through, the vesicular bilayers.
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| Yaroslavov, Alexander A; Sybachin, Andrei V; Schrinner, Marc et al. (2010) Liposomes remain intact when complexed with polycationic brushes. J Am Chem Soc 132:5948-9 |
| Yaroslavov, Alexander A; Sitnikova, Tatiana A; Rakhnyanskaya, Anna A et al. (2009) Biomembrane sensitivity to structural changes in bound polymers. J Am Chem Soc 131:1666-7 |
| Davydov, Dmitry A; Yaroslavova, Ekaterina G; Rakhnyanskaya, Anna A et al. (2009) Polymer migration among phospholipid liposomes. Langmuir 25:13528-33 |
| Yaroslavov, Alexander A; Rakhnyanskaya, Anna A; Yaroslavova, Ekaterina G et al. (2008) Polyelectrolyte-coated liposomes: stabilization of the interfacial complexes. Adv Colloid Interface Sci 142:43-52 |
| Sybachin, A V; Efimova, A A; Litmanovich, E A et al. (2007) Complexation of polycations to anionic liposomes: composition and structure of the interfacial complexes. Langmuir 23:10034-9 |
| Yaroslavov, A A; Sitnikova, T A; Rakhnyanskaya, A A et al. (2007) Contrasting behavior of zwitterionic and cationic polymers bound to anionic liposomes. Langmuir 23:7539-44 |
| Yaroslavov, Alexander A; Melik-Nubarov, Nikolay S; Menger, Fredric M (2006) Polymer-induced flip-flop in biomembranes. Acc Chem Res 39:702-10 |
| Kitaeva, M V; Melik-Nubarov, N S; Menger, F M et al. (2004) Doxorubicin-poly(acrylic acid) complexes: interaction with liposomes. Langmuir 20:6575-9 |
| Menger, Fredric M; Seredyuk, Victor A; Yaroslavov, Alexander A (2002) Adhesive and anti-adhesive agents in giant vesicles. Angew Chem Int Ed Engl 41:1350-2 |
