The long-term objective of the proposed research is to uncover genetic and molecular mechanisms of cell differentiation in the vertebrate retina. Recessive mutations affecting the development of the eye in zebrafish (Danio rerio) have been recovered from a chemical mutagenesis screen, and the applicant proposes to further characterize these mutant lines.
The first aim i s to examine the mutant eyes by histological and immunological analysis, and to select a few lines for further analysis.
The second aim i s to define the nature of the mutated genes by genetic mosaic analysis (transplanting cells from mutant into wildtype embryos) and by complementation analysis (searching for additional alleles of the mutated genes by genetic methods).

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
1R15EY011774-01
Application #
2020279
Study Section
Visual Sciences C Study Section (VISC)
Project Start
1997-07-01
Project End
2000-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Auburn University at Auburn
Department
Zoology
Type
Schools of Arts and Sciences
DUNS #
City
Auburn University
State
AL
Country
United States
Zip Code
36849
Fadool, J M; Hartl, D L; Dowling, J E (1998) Transposition of the mariner element from Drosophila mauritiana in zebrafish. Proc Natl Acad Sci U S A 95:5182-6