Preconditioning induced by repeated brief myocardial ischemia each separated by another brief period of reperfusion protects the myocardium from subsequent ischemia reperfusion injury. One of beneficial consequences of preconditioning is progressive preservation of high energy phosphate compounds which is reflected by the progressively greater attenuation of the increase in interstitial fluid (ISF) purine metabolites. The proposed research will examine the threshold of the attenuated purine metabolite accumulation by addressing two Specific Aims: 1) by determining the minimum time and degree of ischemia necessary to induce attenuated purine metabolite accumulation during subsequent global ischemia and 2) by determining the effect of adenosine kinase or adenosine deaminase inhibition on ISF purine metabolite accumulation and by studying the rate of adenosine formation during preconditioning.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
1R15HL062930-01
Application #
2879389
Study Section
Cardiovascular and Renal Study Section (CVB)
Project Start
1999-09-01
Project End
2002-08-31
Budget Start
1999-09-01
Budget End
2002-08-31
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
St. Olaf College
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
041201070
City
Northfield
State
MN
Country
United States
Zip Code
55057
Chang, Stewart T; Linderman, Jennifer J; Kirschner, Denise E (2005) Multiple mechanisms allow Mycobacterium tuberculosis to continuously inhibit MHC class II-mediated antigen presentation by macrophages. Proc Natl Acad Sci U S A 102:4530-5