Abstract

The principal proposes in this R15 competing renewal to evaluate mechanisms of brain zinc Zn) homeostasis maintenance. Zinc transport will be assessed in an in vitro model of the blood brain barrier (BBB) under different conditions of Zn status. Both brain uptake and brain to blood transport will be determined. The proposal also seeks to evaluate Zn transporter expression through Northern, Western and 2-D PAGE analysis from the in vitro BBB model and by isolating brain capillaries from the brains of minipigs subjected to varying Zn conditions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
2R15NS035285-02
Application #
6160124
Study Section
Special Emphasis Panel (ZRG1-BDCN-3 (01))
Program Officer
Jacobs, Tom P
Project Start
1996-05-01
Project End
2004-07-31
Budget Start
2000-08-06
Budget End
2004-07-31
Support Year
2
Fiscal Year
2000
Total Cost
$144,000
Indirect Cost

  Institution

Name
University of New Hampshire
Department
Veterinary Sciences
Type
Schools of Earth Sciences/Natur
DUNS #
111089470
City
Durham
State
NH
Country
United States
Zip Code
03824

  Publications

Bobilya, Dennis J; Gauthier, Nicole A; Karki, Shakun et al. (2008) Longitudinal changes in zinc transport kinetics, metallothionein and zinc transporter expression in a blood-brain barrier model in response to a moderately excessive zinc environment. J Nutr Biochem 19:129-37
Jeliazkova-Mecheva, Valentina V; Hymer, Wes C; Nicholas, Nicholas C et al. (2006) Brief heat shock affects the permeability and thermotolerance of an in vitro blood-brain barrier model of porcine brain microvascular endothelial cells. Microvasc Res 71:108-14
Jeliazkova-Mecheva, Valentina V; Bobilya, Dennis J (2003) A porcine astrocyte/endothelial cell co-culture model of the blood-brain barrier. Brain Res Brain Res Protoc 12:91-8
Lehmann, Holly M; Brothwell, Barbara B; Volak, Laurie P et al. (2002) Zinc status influences zinc transport by porcine brain capillary endothelial cells. J Nutr 132:2763-8
Rowe, D J; Bobilya, D J (2000) Albumin facilitates zinc acquisition by endothelial cells. Proc Soc Exp Biol Med 224:178-86