Abstract

The overall goal of this application is to develop ELISA spot assays for various molecules secreted by CD8 memory cells after antigen stimulation, in particular for the cytokines, perforin and granzyme-B. The proposed working hypotheses is that, by detecting the antigen/peptide-induced production of these molecules, ELISA spot assays could be used to study antigen-specific CD8 cell memory in freshly isolated cell populations thereby providing a sensitive measurement of antigen-specific CD8 cell activity in vivo. Cytokine ELISA spot measurements should be particularly informative about the differential effector functions of these cells, which may play as important a role in CD8 cell-mediated host defense mere killing does.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21AI042635-02
Application #
2673186
Study Section
Special Emphasis Panel (ZAI1-VSG-A (O2))
Project Start
1997-09-30
Project End
1999-09-29
Budget Start
1998-09-30
Budget End
1999-09-29
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Pathology
Type
Schools of Medicine
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Schlingmann, Tobias R; Rininsland, Frauke H; Bartholomae, Wolf C et al. (2009) Vaccination with tumor cells pulsed with foreign peptide induces immunity to the tumor itself. Clin Immunol 133:45-51
Ott, Patrick A; Herzog, Bernhard A; Quast, Stefan et al. (2005) Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls. Clin Immunol 115:102-14
Ott, Patrick A; Anderson, Michael R; Tary-Lehmann, Magdalena et al. (2005) CD4+CD25+ regulatory T cells control the progression from periinsulitis to destructive insulitis in murine autoimmune diabetes. Cell Immunol 235:1-11
Ott, Patrick A; Dittrich, Marcus T; Herzog, Bernhard A et al. (2004) T cells recognize multiple GAD65 and proinsulin epitopes in human type 1 diabetes, suggesting determinant spreading. J Clin Immunol 24:327-39
Darabi, Kamruz; Karulin, Alexey Y; Boehm, Bernhard O et al. (2004) The third signal in T cell-mediated autoimmune disease? J Immunol 173:92-9
Herzog, Bernhard A; Ott, Patrick A; Dittrich, Marcus T et al. (2004) Increased in vivo frequency of IA-2 peptide-reactive IFNgamma+/IL-4- T cells in type 1 diabetic subjects. J Autoimmun 23:45-54
Guerkov, Robert E M; Targoni, Oleg S; Kreher, Christian R et al. (2003) Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods 279:111-21
Hofstetter, Harald H; Sewell, Diane L; Liu, Frances et al. (2003) Autoreactive T cells promote post-traumatic healing in the central nervous system. J Neuroimmunol 134:25-34
Karulin, Alexey Y; Hesse, Maike D; Yip, Hualin C et al. (2002) Indirect IL-4 pathway in type 1 immunity. J Immunol 168:545-53
Targoni, O S; Baus, J; Hofstetter, H H et al. (2001) Frequencies of neuroantigen-specific T cells in the central nervous system versus the immune periphery during the course of experimental allergic encephalomyelitis. J Immunol 166:4757-64

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