Abstract

Dr. Picker proposes to develop two new assays for quantitating recent thymic emigrants in humans, the detection of T cell receptor rearrangement excision circles and the use of multiparameter flow cytometry to detect unique cell surface phenotypes. The first assay measures very common TC rearrangement-generated episomal circles from the TCR-delta rec locus to pseudo-J-alpha region, and the second assay measures CD8+ T cells that are CD103+, CD27 bright, CD95 dim, CD45RA+/RO-, Cdlla dim, and CD62L bright. When these assays are standardized, they will be applied to patients on HAART to monitor the level of thymic output of naiDve T cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21AI044758-02
Application #
2887953
Study Section
Special Emphasis Panel (ZRG5-AARR-2 (03))
Program Officer
Kagan, Jonathan M
Project Start
1998-09-30
Project End
2000-01-31
Budget Start
1999-09-30
Budget End
2000-01-31
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Pathology
Type
Schools of Medicine
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390

  Publications

Pitcher, Christine J; Hagen, Shoko I; Walker, Joshua M et al. (2002) Development and homeostasis of T cell memory in rhesus macaque. J Immunol 168:29-43
Markert, M L; Hicks, C B; Bartlett, J A et al. (2000) Effect of highly active antiretroviral therapy and thymic transplantation on immunoreconstitution in HIV infection. AIDS Res Hum Retroviruses 16:403-13
McFarland, R D; Douek, D C; Koup, R A et al. (2000) Identification of a human recent thymic emigrant phenotype. Proc Natl Acad Sci U S A 97:4215-20
Evans, J T; Okamoto, Y; Douek, D C et al. (2000) Thymocyte differentiation from lentivirus-marked CD34(+) cells in infant and adult human thymus. J Immunol Methods 245:31-43