We will investigate combined injury by moderate dose radiation in two models in a two-phase study. In the first phase, we will use anonymized surgical samples irradiated and wounded ex vivo by burning to study gene and protein expression differences, develop a non-invasive assay to predict successful wound healing, and test a beta adrenergic receptor antagonist as potential therapy to improve wound healing. In the second phase, we will investigate similar issues in a mouse model. Thus, the total study will combine human data (which is, however, not in vivo) and data from intact animals (which is in vivo) to obtain relevant information that can inform the development of a better mechanistic understanding of the mechanisms of wound healing in the presence of radiation damage, and to develop potential therapies that can later be tested in the clinic. Public Health Relevance In a nuclear warfare or terrorist incident, combined injury by radiation and burn wounding is likely. We will provide a better mechanistic understanding of the molecular biology behind healing of combined injury wounds, and will test a non-invasive assay for wound healing success and a potential therapy to improve healing. All of this would be of considerable public health benefit in such a scenario. ? ? ?

Public Health Relevance

In a nuclear warfare or terrorist incident, combined injury by radiation and burn wounding is likely. We will provide a better mechanistic understanding of the molecular biology behind healing of combined injury wounds, and will test a non-invasive assay for wound healing success and a potential therapy to improve healing. All of this would be of considerable public health benefit in such a scenario.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21AI080604-01
Application #
7568561
Study Section
Special Emphasis Panel (ZAI1-BDP-I (M3))
Program Officer
Dicarlo-Cohen, Andrea L
Project Start
2008-07-15
Project End
2010-06-30
Budget Start
2008-07-15
Budget End
2009-06-30
Support Year
1
Fiscal Year
2008
Total Cost
$190,000
Indirect Cost
Name
University of California Davis
Department
Public Health & Prev Medicine
Type
Schools of Medicine
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Albrecht, Huguette; Yang, Hsin-Ya; Kiuru, Maija et al. (2018) The Beta 2 Adrenergic Receptor Antagonist Timolol Improves Healing of Combined Burn and Radiation Wounds. Radiat Res 189:441-445
Oskarsson, Björn; Rocke, David M; Dengel, Karsten et al. (2016) Myasthenia gravis exacerbation after discontinuing mycophenolate: A single-center cohort study. Neurology 86:1159-63
Xia, Jing; Rocke, David M; Perry, George et al. (2014) Differential network analyses of Alzheimer's disease identify early events in Alzheimer's disease pathology. Int J Alzheimers Dis 2014:721453
Babalola, Olubukola; Mamalis, Andrew; Lev-Tov, Hadar et al. (2014) NADPH oxidase enzymes in skin fibrosis: molecular targets and therapeutic agents. Arch Dermatol Res 306:313-330
Dasu, Mohan R; Ramirez, Sandra R; La, Thi Dinh et al. (2014) Crosstalk between adrenergic and toll-like receptors in human mesenchymal stem cells and keratinocytes: a recipe for impaired wound healing. Stem Cells Transl Med 3:745-59
Babalola, Olubukola; Mamalis, Andrew; Lev-Tov, Hadar et al. (2014) Optical coherence tomography (OCT) of collagen in normal skin and skin fibrosis. Arch Dermatol Res 306:1-9
Albrecht, Huguette; Durbin-Johnson, Blythe; Yunis, Reem et al. (2012) Transcriptional response of ex vivo human skin to ionizing radiation: comparison between low- and high-dose effects. Radiat Res 177:69-83
Yunis, Reem; Albrecht, Huguette; Kalanetra, Karen M et al. (2012) Genomic characterization of a three-dimensional skin model following exposure to ionizing radiation. J Radiat Res 53:860-75
Ray, Monika; Yunis, Reem; Chen, Xiucui et al. (2012) Comparison of low and high dose ionising radiation using topological analysis of gene coexpression networks. BMC Genomics 13:190
Rizzo, Amilcar Ezequiel; Beckett, Laurel A; Baier, Brian S et al. (2012) The linear excisional wound: an improved model for human ex vivo wound epithelialization studies. Skin Res Technol 18:125-32

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