There is a great interest in imaging neuronal activity based on changes in fast intrinsic optical signals (e.g. changes in light scattering and phase) that occur on a millisecond timescale. Fast intrinsic optical signals are related to alteration in the complex refractive index and small volume changes near the neuron membrane, in response to the rapid osmotic changes associated with ion fluxes during action potentials. Optical coherence tomography (OCT) is an emerging biomedical imaging technology that provides label-free and depth-resolved images with micron-scale spatial resolution and sub-millisecond temporal resolution. OCT relies on detection of intrinsic optical contrast, eliminating the need for potentially toxic exogenous contrast agents or genetically- encoded indicators. OCT achieves over 100 dB sensitivity, enabling it to detect weak scattering changes associated with neuronal activity. In addition, OCT has extremely good phase sensitivity (

Public Health Relevance

We plan to develop and validate a label-free and ultrafast optical imaging technology to record millisecond timescale activities associated with action potentials with single neuron resolution. This technology can be used to investigate behavior of thousands of neurons in a network simultaneously, with the potential to significantly impact fundamental brain research.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21EY026380-01
Application #
9055841
Study Section
Special Emphasis Panel (ZEY1)
Program Officer
Wujek, Jerome R
Project Start
2015-09-30
Project End
2017-08-31
Budget Start
2015-09-30
Budget End
2016-08-31
Support Year
1
Fiscal Year
2015
Total Cost
Indirect Cost
Name
Lehigh University
Department
Engineering (All Types)
Type
Biomed Engr/Col Engr/Engr Sta
DUNS #
808264444
City
Bethlehem
State
PA
Country
United States
Zip Code
18015
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Huang, Hao; Huang, Yongyang; Lau, Willie et al. (2018) Integrating optical coherence tomography with gravimetric and video analysis (OCT-Gravimetry-Video method) for studying the drying process of polystyrene latex system. Sci Rep 8:12962
Shi, Wentao; Kwon, Jean; Huang, Yongyang et al. (2018) Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity. Sci Rep 8:6837
Huang, Yongyang; Wang, Shunqiang; Guo, Qiongyu et al. (2017) Optical Coherence Tomography Detects Necrotic Regions and Volumetrically Quantifies Multicellular Tumor Spheroids. Cancer Res 77:6011-6020
Huang, Yongyang; Badar, Mudabbir; Nitkowski, Arthur et al. (2017) Wide-field high-speed space-division multiplexing optical coherence tomography using an integrated photonic device. Biomed Opt Express 8:3856-3867
Wan, Sunhua; Lee, Hsiang-Chieh; Huang, Xiaolei et al. (2017) Integrated local binary pattern texture features for classification of breast tissue imaged by optical coherence microscopy. Med Image Anal 38:104-116
Men, Jing; Huang, Yongyang; Solanki, Jitendra et al. (2016) Optical Coherence Tomography for Brain Imaging and Developmental Biology. IEEE J Sel Top Quantum Electron 22: