Regulation of gene expression at the translation level is important for many biological processes General procedures for identifying and characterizing those mRNA species that are under translational control are needed. In this project, cytosolic mRNAs will be separated into translated and untranslated species by sucrose gradient centrifugation. Radioactive cDNA copies will then be prepared from these mRNA species and used to probe high density cDNA arrays in order to identify those mRNAs that are repressed or are actively being translated. This procedure will then be tested in defining the constellation of genes that are under translational control in response to growth signals in a fibroblast cell line.
| Anthony, C; Zong, Q; De Benedetti, A (2001) Overexpression of eIF4E in Saccharomyces cerevisiae causes slow growth and decreased alpha-factor response through alterations in CLN3 expression. J Biol Chem 276:39645-52 |
| Zong, Q; Schummer, M; Hood, L et al. (1999) Messenger RNA translation state: the second dimension of high-throughput expression screening. Proc Natl Acad Sci U S A 96:10632-6 |
