Studies on the seasonal abundance and distribution of Vibrio and related species from a water supply in a cholera endemic area (Bangladesh) will be continued. The ecological studies will include examination of water, sediment, flora and plankton samples collected at appropriate intervals of time for determining the seasonal abundance and distribution of Vibrio spp. Isolated strains will be subjected to extensive numerical analysis, examined for enteropathogenicity and antibiotic resistance patterns, and analyzed for presence of extrachromosomal genetic elements. The relationship of non-01 strains of V. cholerae isolated from the water supply and from the Chesapeake Bay to classical and El Tor V. cholerae will be studied by systematic analysis and DNA/DNA hybridization studies. Analysis of cytotoxic and enterotoxic factors of non-01 vibrios, of V. parahaemolyticus and of Aeromonas hydrophila will be pursued on both an ecological and molecular genetic basis. Correlations will be sought between individual strain characteristics and presence of extrachromosomal elements, and factors relating to pathogenicity and natural distribution. By using a computer based system, development of an on-line identification system for vibrio species will be developed.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
5R22AI014242-09
Application #
3444475
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1977-07-01
Project End
1989-03-31
Budget Start
1987-07-01
Budget End
1989-03-31
Support Year
9
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of Maryland College Park
Department
Type
Earth Sciences/Resources
DUNS #
City
College Park
State
MD
Country
United States
Zip Code
20742
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Thomasian, S K; Voll, M J (1989) Specificity of transposition of Tn7 in Vibrio parahaemolyticus. Plasmid 22:82-5
Brayton, P R; Tamplin, M L; Huq, A et al. (1987) Enumeration of Vibrio cholerae O1 in Bangladesh waters by fluorescent-antibody direct viable count. Appl Environ Microbiol 53:2862-5
O'Brien, M; Colwell, R R (1987) A rapid test for chitinase activity that uses 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide. Appl Environ Microbiol 53:1718-20
Huq, A; Huq, S A; Grimes, D J et al. (1986) Colonization of the gut of the blue crab (Callinectes sapidus) by Vibrio cholerae. Appl Environ Microbiol 52:586-8
Voll, M J; Colwell, R R; Maneval, D (1986) Characterization of non-01 Vibrio cholerae and V. mimicus isolated from fresh water in Bangladesh. J Diarrhoeal Dis Res 4:216-21
Grimes, D J; Atwell, R W; Brayton, P R et al. (1986) The fate of enteric pathogenic bacteria in estuarine and marine environments. Microbiol Sci 3:324-9