Abstract

The alphavirus Sindbis will serve as a model for studies on the processes involved in the assembly of enveloped viruses. Information will be gathered on the processing of virus membrane proteins and the interactions of virus proteins with other virus proteins and host components as the virus proteins are moved from their sight of synthesis to the cell surface. The final stages in the envelopment of the virus will be studied to determine the topological arrangement of precursors to the virus envelope glycoproteins in the infected cell plasma membrane and the reorganization of these virus proteins as building of the virion takes place. Sindbis virus capsid protein-RNA complexes will be examined in experiments determining the nature of RNA-protein and protein-protein interactions involved in the assembly of the icosahedral capsid. Morphological rearrangements occurring in the capsid during envelopment will be investigated. The development of functional capacities of virus membrane proteins (attachment and membrane fusion) will be examined.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
5R22AI014710-07
Application #
3444478
Study Section
Experimental Virology Study Section (EVR)
Project Start
1978-09-01
Project End
1988-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Texas Austin
Department
Type
Graduate Schools
DUNS #
City
Austin
State
TX
Country
United States
Zip Code
78713

  Publications

Carleton, M; Brown, D T (1997) The formation of intramolecular disulfide bridges is required for induction of the Sindbis virus mutant ts23 phenotype. J Virol 71:7696-703
Karpf, A R; Lenches, E; Strauss, E G et al. (1997) Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus. J Virol 71:7119-23
Carleton, M; Lee, H; Mulvey, M et al. (1997) Role of glycoprotein PE2 in formation and maturation of the Sindbis virus spike. J Virol 71:1558-66
Lee, S; Owen, K E; Choi, H K et al. (1996) Identification of a protein binding site on the surface of the alphavirus nucleocapsid and its implication in virus assembly. Structure 4:531-41
Carleton, M; Brown, D T (1996) Events in the endoplasmic reticulum abrogate the temperature sensitivity of Sindbis virus mutant ts23. J Virol 70:952-9
Liu, L N; Lee, H; Hernandez, R et al. (1996) Mutations in the endo domain of Sindbis virus glycoprotein E2 block phosphorylation, reorientation of the endo domain, and nucleocapsid binding. Virology 222:236-46
Carleton, M; Brown, D T (1996) Disulfide bridge-mediated folding of Sindbis virus glycoproteins. J Virol 70:5541-7
Mulvey, M; Brown, D T (1996) Assembly of the Sindbis virus spike protein complex. Virology 219:125-32
Mulvey, M; Brown, D T (1995) Involvement of the molecular chaperone BiP in maturation of Sindbis virus envelope glycoproteins. J Virol 69:1621-7
Rumenapf, T; Brown, D T; Strauss, E G et al. (1995) Aura alphavirus subgenomic RNA is packaged into virions of two sizes. J Virol 69:1741-6

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