The significance of schistosomiasis as a globe-spanning public health problem, and the significance of immunological approaches to its eventual control and elimination, are well known: The most fundamental aspect of dual immunity in schistosomiasis involves the existence of two largely distinct pathways of specific immune induction; leading to acquired (concomitant) immunity on the one hand and granulomatous hypersensitivity to tissue eggs of the parasite on the other. However, certain connections between these two pathways exist, and examination of several of these links constitutes the basis of this proposal. Using primarily nonhuman primates (baboons and tamarins), we propose to investigate the following: 1. the occurrence of egg-induced pathology-associated resistance, as seen in the murine-Schistosoma mansoni system, in nonhuman primate models for human schistosomiasis. The effect of liver size on egg and schistosomula shunting to the lungs (i.e. """"""""liver leakiness"""""""") will be determined by comparing large (baboons) and very small (tamarins) primates. 2. baboons will be vaccinated with irradiated, cryopreserved schistosomula in order to ascertain vaccine effects, including stimulation of acquired immunity, induction of granuloma size reduction and reduction of hypergammaglobulinemia. Protection-associated antibodies will be sought. Further evidence for immunoregulation (modulation) in the granuloma reduction phenomenon will also be sought.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
2R22AI018906-05
Application #
3444593
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1982-04-01
Project End
1988-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
5
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Georgia
Department
Type
Schools of Arts and Sciences
DUNS #
City
Athens
State
GA
Country
United States
Zip Code
30602
Makaaru, C K; Damian, R T; Smith, D F et al. (1992) The human blood fluke Schistosoma mansoni synthesizes a novel type of glycosphingolipid. J Biol Chem 267:2251-7
Nyame, K; Smith, D F; Damian, R T et al. (1989) Complex-type asparagine-linked oligosaccharides in glycoproteins synthesized by Schistosoma mansoni adult males contain terminal beta-linked N-acetylgalactosamine. J Biol Chem 264:3235-43
Nyame, K; Cummings, R D; Damian, R T (1988) Characterization of the N- and O-linked oligosaccharides in glycoproteins synthesized by Schistosoma mansoni schistosomula. J Parasitol 74:562-72
McCormick, S L; Damian, R T (1987) Haptenation of adult Schistosoma mansoni and assessment of humorally mediated damage in vitro. J Parasitol 73:130-43
Damian, R T (1987) Immunological aspects of host-schistosome relationships. Mem Inst Oswaldo Cruz 82 Suppl 4:13-6
Del Portillo, H A; Schmidt, G W; Damian, R T (1987) Immunochemical analysis of baboon (Papio cynocephalus) IgG subclasses. Vet Immunol Immunopathol 16:201-14
Damian, R T (1987) The exploitation of host immune responses by parasites. J Parasitol 73:3-13
Nyame, K; Cummings, R D; Damian, R T (1987) Schistosoma mansoni synthesizes glycoproteins containing terminal O-linked N-acetylglucosamine residues. J Biol Chem 262:7990-5
Zerda, K S; Dresden, M H; Damian, R T et al. (1987) Schistosoma mansoni: anti-SMw32 proteinase response in vaccinated and challenged baboons. Am J Trop Med Hyg 37:320-6
Bosshardt, S C; Damian, R T (1986) Serum factors from infected baboons inhibit oviposition and cause unpairing of Schistosoma mansoni in vitro. J Parasitol 72:583-7

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