Abstract

With the aid of stable isotope tracer methods, involving use of combined gas chromatograph-mass spectrometric techniques, we are exploring the adaptations of whole body protein and amimo acid metabolism in adult subjects. Studies include the effects of level and source of protein, amino acids and energy intake on whole body leucine, lysine and glycine and alanine metabolism. Concurrent use of 13C- and 15N-labels provides an opportunity to examine dynamic aspects and interrelationships among a number of amino acids, for a given dietary condition. These studies have shown that the specific mechanisms for achieving body amino acid homeostasis depend upon the range of protein intake studied; that is, they also appear to be integrated with the nitrogen and amino acid requirements of the individual whether in the submaintenance or surfeit range of intake.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Unknown (R22)
Project #
5R22DK015856-15
Application #
3445078
Study Section
(SSS)
Project Start
1983-09-01
Project End
1987-12-31
Budget Start
1987-01-01
Budget End
1987-12-31
Support Year
15
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Tharakan, John F; Yu, Yong M; Zurakowski, David et al. (2008) Adaptation to a long term (4 weeks) arginine- and precursor (glutamate, proline and aspartate)-free diet. Clin Nutr 27:513-22
Louis, Magali; Poortmans, Jacques R; Francaux, Marc et al. (2003) Creatine supplementation has no effect on human muscle protein turnover at rest in the postabsorptive or fed states. Am J Physiol Endocrinol Metab 284:E764-70
Chen, Chung-Lin; Fei, Zhewei; Carter, Edward A et al. (2003) Metabolic fate of extrahepatic arginine in liver after burn injury. Metabolism 52:1232-9
Argaman, Zvi; Young, Vernon R; Noviski, Natan et al. (2003) Arginine and nitric oxide metabolism in critically ill septic pediatric patients. Crit Care Med 31:591-7
Rand, William M; Pellett, Peter L; Young, Vernon R (2003) Meta-analysis of nitrogen balance studies for estimating protein requirements in healthy adults. Am J Clin Nutr 77:109-27
Yu, Yong-Ming; Ryan, Colleen M; Fei, Zhe-Wei et al. (2002) Plasma L-5-oxoproline kinetics and whole blood glutathione synthesis rates in severely burned adult humans. Am J Physiol Endocrinol Metab 282:E247-58
Young, V R; Ajami, A M (2001) Metabolism 2000: the emperor needs new clothes. Proc Nutr Soc 60:27-44
Lyons, J; Rauh-Pfeiffer, A; Ming-Yu, Y et al. (2001) Cysteine metabolism and whole blood glutathione synthesis in septic pediatric patients. Crit Care Med 29:870-7
Yu, Y M; Ryan, C M; Castillo, L et al. (2001) Arginine and ornithine kinetics in severely burned patients: increased rate of arginine disposal. Am J Physiol Endocrinol Metab 280:E509-17
Forslund, A H; Hambraeus, L; van Beurden, H et al. (2000) Inverse relationship between protein intake and plasma free amino acids in healthy men at physical exercise. Am J Physiol Endocrinol Metab 278:E857-67

Showing the most recent 10 out of 77 publications