We propose to investigate the biology of steroid-receptor-negative breast cancer, using three approaches: (1) We will fuse steroid-receptor-negative and -positive breast cancer cells to see if steroid-receptor-negativity, and its associated phenotype are suppressible (i.e., recessive genotypic characteristics); (2) We will fuse steroid-receptor-negative nuclei (karyoplasts) with steroid-receptor-positive cytoplasm (cytoplasts) to see if the steroid-receptor-negative nuclei can respond to a 17-B-estradiol in the presence of cytosolic steroid receptor; (3) We will use a monoclonal antibody directed against a steroid-receptor-positive human breast cancer cell line to probe the dynamic relationships of steroid-receptor-negative and -positive breast cancer in vitro and in vivo. Completion of these research projects should significantly increase our knowledge of the biology of steroid-receptor-negative breast cancer, a previously little-investigated problem in experimental oncology.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Unknown (R23)
Project #
5R23CA038022-03
Application #
3446581
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1985-04-01
Project End
1988-03-31
Budget Start
1987-04-01
Budget End
1988-03-31
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Indiana University-Purdue University at Indianapolis
Department
Type
Schools of Medicine
DUNS #
005436803
City
Indianapolis
State
IN
Country
United States
Zip Code
46202