Initial studies have shown that DNAs from thymic lymphomas induced in (AKR X RF)F1 mice by chemical carcinogen or radiation treatment contain activated N-ras or activated K-ras oncogenes, respectively. Several strains of mice treated similarly will be surveyed for oncogene activation using the technique of DNA-mediated gene transfer in two assays: (1) an in vitro assay to screen for ras oncogenes and (2) an in vivo assay utilizing nude mice to screen for new oncogenes. The anomalous expression of TL antigen on these tumors precedes the development of overt disease and will be used to follow and fractionate subpopulations of thymocytes from treated mice during the latent period. Immunohistochemistry of normal and leukemic tissue will be used to detect the expression of the ras gene product (p21 protein) and to determine the temporal relationship between ras and TL gene expression during the latent period of tumor development. Thymomas will be analyzed for chromosomal abnormalities, particularly trisomy 15, to identify correlations between specific chromosome changes and changes in gene expression. These studies should further our understanding about the genes involved in the malignant process and at what stages they may act.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Unknown (R23)
Project #
1R23CA040533-01
Application #
3446797
Study Section
Chemical Pathology Study Section (CPA)
Project Start
1985-07-01
Project End
1988-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost
Name
New York University
Department
Type
Schools of Medicine
DUNS #
004514360
City
New York
State
NY
Country
United States
Zip Code
10012