Helicobacters have emerged as a major problem for mouse-based research, directly causing disease, misinterpretation of phenotype in genetically altered animals, and alteration of experimental results. Infection is prevalent and spreading due to inter-institutional traffic of mice. Current diagnostic assays, based upon culture, membrane antigens or polymerase chain reaction, are not practical or amenable to large-scale testing, so there is need to develop cost-effective and efficient assays for testing serum or feces of infected mice. This project will identify immunogenic proteins of Helicobacter hepaticus and Helicobacter bilis by screening genomic expression libraries with immune serum from infected mice, then clone, sequence and express candidate recombinant proteins for development as species- and genus-specific antigens for serodiagnostic assays. The antigens will also be utilized to develop species- and genus-specific monoclonal antibodies that can be used for antigen-capture assays for testing feces. The species- and genus-specificity of these assays will be tested with sera from mice experimentally infected with clonal populations of Helicobacter hepaticus, Helicobacter bilis, Helicobacter muridarum, Flexispira rappini, and Helicobacter rodentium, if available.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Resource-Related Research Projects (R24)
Project #
5R24RR014034-02
Application #
6394719
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Grieder, Franziska B
Project Start
2000-09-30
Project End
2005-08-31
Budget Start
2001-09-01
Budget End
2002-08-31
Support Year
2
Fiscal Year
2001
Total Cost
$277,746
Indirect Cost
Name
University of California Davis
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618
Feng, Sunlian; Ku, Karin; Hodzic, Emir et al. (2005) Differential detection of five mouse-infecting helicobacter species by multiplex PCR. Clin Diagn Lab Immunol 12:531-6
Khan, Imran H; Kendall, Lon V; Ziman, Melanie et al. (2005) Simultaneous serodetection of 10 highly prevalent mouse infectious pathogens in a single reaction by multiplex analysis. Clin Diagn Lab Immunol 12:513-9
Feng, Sunlian; Kendall, Lon V; Hodzic, Emir et al. (2004) Recombinant Helicobacter bilis protein P167 for mouse serodiagnosis in a multiplex microbead assay. Clin Diagn Lab Immunol 11:1094-9
Kendall, Lon V; Feng, Sunlian; Hodzic, Emir et al. (2004) Use of the P167 recombinant antigen for serodiagnosis of Helicobacter bilis. Comp Med 54:44-8
Feng, Sunlian; Hodzic, Emir; Kendall, Lon V et al. (2002) Cloning and expression of a Helicobacter bilis immunoreactive protein. Clin Diagn Lab Immunol 9:627-32
Hodzic, E; McKisic, M; Feng, S et al. (2001) Evaluation of diagnostic methods for Helicobacter bilis infection in laboratory mice. Comp Med 51:406-12