In this proposal, a young investigator wishes to study if proteins involved in T cell activation might be used in the HIV-1 reverse transcription. To facilitate her studies, she has obtained preliminary data demonstrating that T cell proteins bind to biotinylated HIV-1 single stranded DNA and RNA constructs using a non-radioactive adaptation of the electrophoretic mobility shift assay. Using this technique, the investigator proposes to investigate the interaction of HIV-1 reverse transcription intermediates with T cell proteins to test the hypothesis that early and efficient reverse transcription of HIV-1 ssDNA intermediates may assist HIV-1 in the infection process by providing a binding site for host cell proteins involved in pre-integration or integration events. Towards this goal, the investigator proposes the following Specific Aims:
Specific Aim 1 : To delineate the specific sites on HIV-1 RNA, single stranded DNA, RNA DNA hybrid constructs interacting with T cell proteins.
Specific Aim 2 : To investigate interactions between HIV-1 reverse transcription intermediates and the HIV-1 integrase protein

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29AI038114-03
Application #
2672517
Study Section
AIDS and Related Research Study Section 1 (ARRA)
Project Start
1996-07-15
Project End
2001-06-30
Budget Start
1998-07-01
Budget End
1999-06-30
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
State University of New York at Buffalo
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260
Ludwig, Linda B; Ambrus Jr, Julian L; Krawczyk, Kristie A et al. (2006) Human Immunodeficiency Virus-Type 1 LTR DNA contains an intrinsic gene producing antisense RNA and protein products. Retrovirology 3:80