This is a application to identify cellular and viral factors that directly interact with Vif. Initially, three overlapping or complementary approaches will be taken to this end. These include association of proteins with GST-Vif fusion proteins, isolation of cDNAs that encode proteins that interact with Vif in a yeast two hybrid screen, and coimmunoprecipitation and crosslinking studies. Candidate proteins will be examined further by screening with many Vif mutants to find mutants that reduce or eliminate binding or association of the two proteins. Such mutations will then examined in depth for their effect on replication and specific properties of Vif.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29AI039972-04
Application #
2887222
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Program Officer
Plaeger, Susan F
Project Start
1996-09-01
Project End
2001-08-31
Budget Start
1999-09-01
Budget End
2000-08-31
Support Year
4
Fiscal Year
1999
Total Cost
Indirect Cost
Name
St. Luke's Roosevelt Hosp Center (New York)
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10025