The P.I. has established a cell-free system for the assembly of immature HIV-1 capsids that resemble immature particles by several biochemical and morphological criteria. Particle assembly in the system appears to proceed through several discrete intermediates and has been shown to be dependent on ATP, myristylation, and a cellular factor. In addition, antibody against the eukaryotic cytosolic chaperonin TCP-1 has been found to recognize Gag proteins in the cell-free lysates, suggesting that a TCP-1 related protein is associated with assembly intermediates. The P.I. proposes to use the system to study the mechanism of viral RNA encapsidation (AIM #1) and to define the role of assembly intermediates (AIM #2).
Aim #3 is to purify the TCP-1 related protein in order to obtain high affinity antibodies and functional proteins that can be used in inactivation, depletion and reconstitution experiments that define the TCP-1 role.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29AI041881-06
Application #
6373703
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Program Officer
Sharma, Opendra K
Project Start
1997-09-01
Project End
2002-08-31
Budget Start
2001-09-01
Budget End
2002-08-31
Support Year
6
Fiscal Year
2001
Total Cost
$63,215
Indirect Cost
Name
University of Washington
Department
Pathology
Type
Schools of Public Health
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195