Oncogenes have, in many cases, been shown to interfere with as well as growth regulation. Very little is known, however, about differentiation the relationship between these oncogene functions. The goal of the proposed project is to determine the mechanism of SV40 large T antigen in a well defined, novel assay for differentiation. In order to determine, at a molecular level, how T antigen blocks differentiation we will perform the following experiments: 1. A detailed structure-function analysis of SV40 T antigen block of adipocyte differentiation using existing mutants. 2. Determine whether Sv40 T antigen blocks adipocyte by differentiation interfering with growth factor regulated cell cycle arrest. 3. Determine how SV40 T antigen suppresses the expression of an adipocyte-specific gene family.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29CA044761-03
Application #
3458120
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1989-07-01
Project End
1994-06-30
Budget Start
1991-07-01
Budget End
1992-06-30
Support Year
3
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Tufts University
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02111