The overall objective of this project is to study the molecular mechanisms by which the naturally occuring polyamines, spermidine and spermine, regulate growth gene transcription in human cancer. This proposal seeks to elucidate the mechanism(s) by which the polyamines can influence c-myc transcription by (1) determining the structure-function relationships for polyamines and their interaction with c-myc transcription; (2) exploring how the polyamines specifically influence the chromatin structure and protein binding to the c-myc gene; and (3) examining the role of polyamines in modulation of c-myc transcription in a cell free in vitro transcription system.
| Celano, P; Vertino, P M; Casero Jr, R A (1993) Isolation of polyadenylated RNA from cultured cells and intact tissues. Biotechniques 15:26-8 |
| Celano, P; Berchtold, C M; Mabry, M et al. (1993) Induction of markers of normal differentiation in human colon carcinoma cells by the v-rasH oncogene. Cell Growth Differ 4:341-7 |
| Celano, P; Berchtold, C M; Kizer, D L et al. (1992) Characterization of an endogenous RNA transcript with homology to the antisense strand of the human c-myc gene. J Biol Chem 267:15092-6 |
| Casero Jr, R A; Celano, P; Ervin, S J et al. (1991) Isolation and characterization of a cDNA clone that codes for human spermidine/spermine N1-acetyltransferase. J Biol Chem 266:810-4 |
