Abstract

Gene therapy is a conceptually new approach for the treatment of human disease. It requires a carrier to deliver to the target cells a piece of DNA containing genes that are effective in manipulating cellular function. In this project, we propose to develop an emulsion based carrier system for gene delivery. The main objective of this proposal is (1) to optimize delivery efficiency of emulsion-based carrier system; (2) to study the mechanisms involved In emulsion-mediated gene delivery; and (3) to develop target-specific, emulsion-based gene carrier system.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29CA072925-02
Application #
2654251
Study Section
Experimental Therapeutics Subcommittee 1 (ET)
Program Officer
Wolpert, Mary K
Project Start
1997-02-01
Project End
2002-01-31
Budget Start
1998-02-01
Budget End
1999-01-31
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Pharmacology
Type
Schools of Pharmacy
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Zhou, Tian; Kamimura, Kenya; Zhang, Guisheng et al. (2010) Intracellular gene transfer in rats by tail vein injection of plasmid DNA. AAPS J 12:692-8
Kamimura, Kenya; Zhang, Guisheng; Liu, Dexi (2010) Image-guided, intravascular hydrodynamic gene delivery to skeletal muscle in pigs. Mol Ther 18:93-100
Kamimura, Kenya; Suda, Takeshi; Xu, Wei et al. (2009) Image-guided, lobe-specific hydrodynamic gene delivery to swine liver. Mol Ther 17:491-9
Kamimura, Kenya; Liu, Dexi (2008) Physical approaches for nucleic acid delivery to liver. AAPS J 10:589-95
Song, Young K; Liu, Feng; Zhang, Guisheng et al. (2002) Hydrodynamics-based transfection: simple and efficient method for introducing and expressing transgenes in animals by intravenous injection of DNA. Methods Enzymol 346:92-105
Liu, D; Knapp, J E (2001) Hydrodynamics-based gene delivery. Curr Opin Mol Ther 3:192-7
Ren, T; Zhang, G; Liu, D (2001) Synthesis of galactosyl compounds for targeted gene delivery. Bioorg Med Chem 9:2969-78
Zhang, G; Song, Y K; Liu, D (2000) Long-term expression of human alpha1-antitrypsin gene in mouse liver achieved by intravenous administration of plasmid DNA using a hydrodynamics-based procedure. Gene Ther 7:1344-9
Ren, T; Zhang, G; Liu, F et al. (2000) Synthesis and evaluation of vitamin D-based cationic lipids for gene delivery in vitro. Bioorg Med Chem Lett 10:891-4
Ren, T; Song, Y K; Zhang, G et al. (2000) Structural basis of DOTMA for its high intravenous transfection activity in mouse. Gene Ther 7:764-8

Showing the most recent 10 out of 15 publications