Abstract

The goal of this proposal is to understand the role of CD34 in hematopoiesis through molecular/biochemical means.
Specific Aim 1 is to establish at what stage(s) of progenitor cell commitment and in which myeloid/erythroid lineages CD34 can regulate differentiation in vitro.
Specific Aim 2 is to determine the significance of the intracellular regions of the full- length and truncated CD34 proteins. As a future study, the Applicant will evaluate the effect of over expression of CD34 on developmental hematopoiesis in vivo. The testing hypothesis is that inappropriate expression of CD34 may inhibit or negatively regulate the differentiation of hematopoietic progenitor cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29DK048374-03
Application #
2668315
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1996-03-01
Project End
2001-02-28
Budget Start
1998-03-01
Budget End
1999-02-28
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Felschow, D M; McVeigh, M L; Hoehn, G T et al. (2001) The adapter protein CrkL associates with CD34. Blood 97:3768-75