This application focuses on assembly of regulatory nucleoprotein complex at cAMP-regulated enhancer (CRE). The investigation proposes studies on: 1) regulation of CREB dimerization by accessory proteins initially define role of dimerization in DNA binding and the role of HIV-1 (Tax) or GroEL; 2) the dynamics of coactivator proteins association with phosphorylated forms of CREB; and 3) allosteric modulation of CREB and CBP (a coactivator).
| Lu, Qing; Hutchins, Amanda E; Doyle, Colleen M et al. (2003) Acetylation of cAMP-responsive element-binding protein (CREB) by CREB-binding protein enhances CREB-dependent transcription. J Biol Chem 278:15727-34 |
| Madison, Dana L; Yaciuk, Peter; Kwok, Roland P S et al. (2002) Acetylation of the adenovirus-transforming protein E1A determines nuclear localization by disrupting association with importin-alpha. J Biol Chem 277:38755-63 |
| Lundblad, J R; Kwok, R P; Laurance, M E et al. (1998) The human T-cell leukemia virus-1 transcriptional activator Tax enhances cAMP-responsive element-binding protein (CREB) binding activity through interactions with the DNA minor groove. J Biol Chem 273:19251-9 |
