A molecular cytological approach to the structure and function of the synaptonemal complex is designed to address questions of how the DNA of eurkaryotic cells is organized into chromosomes and of how chromosomes pair, synapse, recombine, and segregate in meiosis. Recently, chromosome organization and behavior during meiotic prophase in the yeast Saccharomyces cerevisiae has been shown cytologically to be very similar to that in mammals, indicating evolutionary conservation in the underlying molecular mechanisms and allowing use of the sophisticated genetics and molecular genetics of yeast for examining the structural basis of the axial organization of chromosomes.
The specific aims of this proposal are to identify and characterize the function of components of the synaptonemal complex in yeast, using a two-pronged approach. An immunocytological screen for antibodies that label the yeast synaptonemal complex will provide probes to be used to select clones from expression libraries. A screen for new classes of mutations will provide a complementary approach to identify genes that function in synaptonemal complex assembly, activates, and/or disassembly. The cytological, genetic, and molecular genetic analyses are expected to complement one another directly and to provide probes and ideas that will then be applied to questions of chromosome organization, behavior and transmission in mammals.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29GM045250-03
Application #
3468283
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1991-01-01
Project End
1995-12-31
Budget Start
1993-01-01
Budget End
1993-12-31
Support Year
3
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Oklahoma Medical Research Foundation
Department
Type
DUNS #
937727907
City
Oklahoma City
State
OK
Country
United States
Zip Code
73104