Papillomaviruses are important human pathogens whose biology is still incompletely understood. This grant is focused on unraveling the repertoire of proteins required for papillomavirus DNA replication in vitro. Reconstituting viral DNA synthesis in vitro has been used before as an invaluable means of understanding the host's replication machinery. Such experiments with SV40 have provided tremendous insight into the basic DNA replication apparatus within mammalian cells. The repertoire of purified proteins sufficient to direct SV40 DNA snthesis in vitro is not sufficient to direct papillomavirus DNA synthesis in vitro. The major goal of this grant is to isolate and characterize the remaining celllular activities required for papillomavirus DNA replication.
| King, Lauren E; Fisk, John C; Dornan, Edward S et al. (2010) Human papillomavirus E1 and E2 mediated DNA replication is not arrested by DNA damage signalling. Virology 406:95-102 |
| Clower, Randolph V; Fisk, John C; Melendy, Thomas (2006) Papillomavirus E1 protein binds to and stimulates human topoisomerase I. J Virol 80:1584-7 |
| Narahari, Janaki; Fisk, John C; Melendy, Thomas et al. (2006) Interactions of the cellular CCAAT displacement protein and human papillomavirus E2 protein with the viral origin of replication can regulate DNA replication. Virology 350:302-11 |
| Hu, Yan; Clower, Randolph V; Melendy, Thomas (2006) Cellular topoisomerase I modulates origin binding by bovine papillomavirus type 1 E1. J Virol 80:4363-71 |
| Clower, Randolph V; Hu, Yan; Melendy, Thomas (2006) Papillomavirus E2 protein interacts with and stimulates human topoisomerase I. Virology 348:13-8 |
