This is a revised application for a FIRST Award which proposes to study the role of phosphatidylinositol 3-kinase (PI3K) in platelet signal transduction. PI3K is a cytosolic lipid kinase that phosphorylates the D-3 hydroxyl position of the inositide ring of PI, leading to the formation of 3- phospho-PI's. Little is known about the role of this enzyme in platelet signal-response coupling. The P.I. proposes to study this problem first by determining how thrombin-induced PI3K activation plays a functional role in the platelet responses of secretion and aggregation. Second, studies are proposed to identify the cytoskeletal proteins and kinases that interact with PI3K and to determine the nature of these interactions. Finally, based on earlier observations by others, experiments will be performed to determine whether the small GTP-binding protein, rhoA, associates with and regulates PI3K activity in platelets.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
1R29HL051413-01A2
Application #
2228145
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1995-08-01
Project End
1999-07-31
Budget Start
1995-08-01
Budget End
1996-07-31
Support Year
1
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Bloodcenter of Wisconsin, Inc.
Department
Type
DUNS #
City
Milwaukee
State
WI
Country
United States
Zip Code
53233