In this application, Dr. Choi proposes to study the mechanism of hepatic lipase action, particularly lipoprotein removal. Hepatic lipase enhances lipoprotein uptake by lipolysis and forming a bridge between apo B of the particle and proteoglycans (HSPG) of the cell surface. Initial studies show that nonenzymatic bridging likely mediates uptake of apo B-containing lipoproteins (LDL and chylomicron remnants). Published studies showed that lipoprotein lipase (LPL) interacts with apoB via protein-protein interactions. Biochemical and cellular experiments are proposed to elucidate interactions between HL, lipoproteins and HSPG.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29HL058034-04
Application #
6184143
Study Section
Metabolism Study Section (MET)
Program Officer
Serrano, Jose
Project Start
1997-04-01
Project End
2002-03-31
Budget Start
2000-04-01
Budget End
2002-03-31
Support Year
4
Fiscal Year
2000
Total Cost
$99,466
Indirect Cost
Name
Palo Alto Medical Foundation Research Institute
Department
Type
DUNS #
622276137
City
Palo Alto
State
CA
Country
United States
Zip Code
94301