Abstract

Public Health Relevance

A bioterror attack could introduce an unknown pathogen into an area, causing disease without a familiar diagnosis. Protecting and treating military and civilian personnel requires rapid disease diagnosis. This proposal describes the design, construction, and testing of a novel diagnostic for concurrent detection of multiple pathogens in a setting where no specialized equipment or expertise is required.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants Phase II (R33)
Project #
3R33AI100190-04S1
Application #
9214514
Study Section
Special Emphasis Panel (NSS)
Program Officer
Repik, Patricia M
Project Start
2012-06-01
Project End
2017-08-31
Budget Start
2016-03-01
Budget End
2016-08-31
Support Year
4
Fiscal Year
2016
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code

  Publications

Myhrvold, Cameron; Freije, Catherine A; Gootenberg, Jonathan S et al. (2018) Field-deployable viral diagnostics using CRISPR-Cas13. Science 360:444-448
Muffat, Julien; Li, Yun; Omer, Attya et al. (2018) Human induced pluripotent stem cell-derived glial cells and neural progenitors display divergent responses to Zika and dengue infections. Proc Natl Acad Sci U S A 115:7117-7122
Bosch, Irene; de Puig, Helena; Hiley, Megan et al. (2017) Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum. Sci Transl Med 9:
Li, Yun; Muffat, Julien; Omer, Attya et al. (2017) Induction of Expansion and Folding in Human Cerebral Organoids. Cell Stem Cell 20:385-396.e3
Tam, Justina O; de Puig, Helena; Yen, Chun-Wan et al. (2017) A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays. J Immunoassay Immunochem 38:355-377
de Puig, Helena; Bosch, Irene; Gehrke, Lee et al. (2017) Challenges of the Nano-Bio Interface in Lateral Flow and Dipstick Immunoassays. Trends Biotechnol 35:1169-1180
Metsky, Hayden C; Matranga, Christian B; Wohl, Shirlee et al. (2017) Zika virus evolution and spread in the Americas. Nature 546:411-415
Sánchez-Purrà, Maria; Carré-Camps, Marc; de Puig, Helena et al. (2017) Surface-Enhanced Raman Spectroscopy-Based Sandwich Immunoassays for Multiplexed Detection of Zika and Dengue Viral Biomarkers. ACS Infect Dis 3:767-776
de Puig, Helena; Bosch, Irene; Carré-Camps, Marc et al. (2017) Effect of the Protein Corona on Antibody-Antigen Binding in Nanoparticle Sandwich Immunoassays. Bioconjug Chem 28:230-238
Pardee, Keith; Green, Alexander A; Takahashi, Melissa K et al. (2016) Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell 165:1255-1266

Showing the most recent 10 out of 13 publications