The purpose of this proposal is to develop innovative strategies for the detection of multiple gene targets in single tumorous cells. The strategy is to use multicolored detection derived from oligonucleotide probes and combined with deconvolution imaging microscopy to use the entire nucleus of the cell as a chip to display hundreds of gene expression targets. The procedure will then be applied to a colorectal cancer cell model first using human cell lines and proceeding to mouse and eventually human tissue. The benefits of this approach are that many genes can be interrogated for their level of expression in every cell within a tissue, thereby deriving important prognostic and diagnostic information from a single probe cocktail.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory/Developmental Grants Phase II (R33)
Project #
5R33CA083208-03
Application #
6377509
Study Section
Special Emphasis Panel (ZCA1-SRRB-C (M3))
Project Start
1999-08-11
Project End
2003-07-31
Budget Start
2001-08-01
Budget End
2002-07-31
Support Year
3
Fiscal Year
2001
Total Cost
$892,304
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461
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