Abstract

The abilities of malignant tumor cells to invade adjacent tissues and spread via the blood to implant, invade and form secondary metastases at distant host sites are determined by both tumor and host properties. Using selected animal and human metastatic models as well as clinical biopsy samples consisting of primary tumors and their metastases, we will continue ongoing work on identifying, isolating, characterizing and developing useful diagnostic assays for metastasis-associated cell surface glycoproteins, proteoglycans, enzymes and antigens. In addition, we will examine gene expression in metastatic and nonmetastatic cells and identify genes which are differentially expressed as well as study the relationship between oncogene insertion, expression and tumor diversification mechanisms. We will also examine certain host properties which may be important in blood-borne metastasis such as organ specific determinants on endothelial cells. Using our recent techniques for isolating organ microvascular endothelial cells, the interactions of various metastatic cells with endothelial cells should allow us to determine the role of specific endothelial components in these processes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Unknown (R35)
Project #
5R35CA044352-03
Application #
3479540
Study Section
(SRC)
Project Start
1987-09-01
Project End
1994-08-31
Budget Start
1989-09-01
Budget End
1990-08-31
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of Texas MD Anderson Cancer Center
Department
Type
Hospitals
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Cavanaugh, P G; Jia, L; Zou, Y et al. (1999) Transferrin receptor overexpression enhances transferrin responsiveness and the metastatic growth of a rat mammary adenocarcinoma cell line. Breast Cancer Res Treat 56:203-17
Yun, Z; Smith, T W; Menter, D G et al. (1997) Differential adhesion of metastatic RAW117 large-cell lymphoma cells under static or hydrodynamic conditions: role of integrin alpha(v) beta3. Clin Exp Metastasis 15:3-11
Yun, Z; Menter, D G; Nicolson, G L (1996) Involvement of integrin alphavbeta3 in cell adhesion, motility, and liver metastasis of murine RAW117 large cell lymphoma. Cancer Res 56:3103-11
Nicolson, N L; Talpaz, M; Nicolson, G L (1996) Chromatin nucleoprotein complexes containing tightly bound c-abl, p53 and bcl-2 gene sequences: correlation with progression of chronic myelogenous leukemia. Gene 169:173-8
Smith, T W; Menter, D G; Nicholson, G L et al. (1996) Regulation of melanoma cell adhesion stabilization to fibronectin. Melanoma Res 6:351-62
Toh, Y; Pencil, S D; Nicolson, G L (1995) Analysis of the complete sequence of the novel metastasis-associated candidate gene, mta1, differentially expressed in mammary adenocarcinoma and breast cancer cell lines. Gene 159:97-104
Nicolson, G L (1995) Tumor cell interactions with the vascular endothelium and their role in cancer metastasis. EXS 74:123-56
Menter, D G; Fitzgerald, L; Patton, J T et al. (1995) Human melanoma integrins contribute to arrest and stabilization potential while flowing over extracellular matrix. Immunol Cell Biol 73:575-83
Mohanam, S; Wang, S W; Rayford, A et al. (1995) Expression of tissue inhibitors of metalloproteinases: negative regulators of human glioblastoma invasion in vivo. Clin Exp Metastasis 13:57-62
Nicolson, N L; Talpaz, M; Nicolson, G L (1995) Interferon-alpha directly inhibits DNA polymerase activity in isolated chromatin nucleoprotein complexes: correlation with IFN-alpha treatment outcome in patients with chronic myelogenous leukemia. Gene 159:105-11

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