Circadian rhythms represent a common form of cellular and organismal regulation seen in most living things; understanding clock function and output are of significant medical importance. Neurospora has been used as a model system to study circadian rhythms for over 40 years. This Equipment Supplement will support the purchase of a new CCD camera and dedicated incubator for collection of time series bioluminescence data in Neurospora. The camera- incubator set-up will be shared by two NIGMS-supported investigators whose MIRA grants focus exclusively on circadian rhythms. Collection of luciferase data underlies nearly all research of circadian rhythms in Neurospora and is essential for most of the work supported by MIRA grants to the two sponsor labs who collectively pioneered this technology for fungi.
Biological clocks work in most cells, including those of fungi and humans, to regulate metabolism. By studying cells of a fungus with a CCD camera that can acquire bioluminescence reporter data, we can understand how clock control works, and how clock malfunction leads to diseases like diabetes and mental illness.
| Chen, Shan; Fuller, Kevin K; Dunlap, Jay C et al. (2018) Circadian Clearance of a Fungal Pathogen from the Lung Is Not Based on Cell-intrinsic Macrophage Rhythms. J Biol Rhythms 33:99-105 |
| Zhou, Xiaoying; Wang, Bin; Emerson, Jillian M et al. (2018) A HAD family phosphatase CSP-6 regulates the circadian output pathway in Neurospora crassa. PLoS Genet 14:e1007192 |
| Fuller, Kevin K; Dunlap, Jay C; Loros, Jennifer J (2018) Light-regulated promoters for tunable, temporal, and affordable control of fungal gene expression. Appl Microbiol Biotechnol 102:3849-3863 |
| Hughes, Michael E; Abruzzi, Katherine C; Allada, Ravi et al. (2017) Guidelines for Genome-Scale Analysis of Biological Rhythms. J Biol Rhythms 32:380-393 |
| Dunlap, Jay C; Loros, Jennifer J (2017) Making Time: Conservation of Biological Clocks from Fungi to Animals. Microbiol Spectr 5: |
| Fuller, K K; Dunlap, J C; Loros, J J (2016) Fungal Light Sensing at the Bench and Beyond. Adv Genet 96:1-51 |
| Dunlap, Jay C; Loros, Jennifer J (2016) Yes, circadian rhythms actually do affect almost everything. Cell Res 26:759-60 |
| Hurley, Jennifer M; Loros, Jennifer J; Dunlap, Jay C (2016) The circadian system as an organizer of metabolism. Fungal Genet Biol 90:39-43 |
| Fuller, Kevin K; Cramer, Robert A; Zegans, Michael E et al. (2016) Aspergillus fumigatus Photobiology Illuminates the Marked Heterogeneity between Isolates. MBio 7: |
| Dasgupta, Arko; Fuller, Kevin K; Dunlap, Jay C et al. (2016) Seeing the world differently: variability in the photosensory mechanisms of two model fungi. Environ Microbiol 18:5-20 |
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