Abstract

My group has a long?standing interest in understanding the molecular mechanisms that contribute to cancer biology and genome integrity. To provide new insights into the mechanisms of genome integrity we have pioneered the development of DNA curtains, which allow for the direct visualization of hundreds to thousands of individual molecules in real?time by optical microscopy. This experimental platform is applicable to many types of protein?nucleic acid interactions and allows us to address questions about complex biological problems that cannot be addressed through traditional approaches. Our success in with this technology has uniquely positioned the lab to reveal new concepts regarding the mechanisms of genome integrity

Public Health Relevance

Double-stranded DNA breaks (DSBs) can cause gross chromosomal rearrangements, which are the hallmark of all forms of cancer. The overall goal of our research is to study the events that lead to DSB formation and the mechanisms that cells use for DSB repair, in the hope that this research will eventually lead to a better understanding of how might be able to prevent or cure cancer

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Unknown (R35)
Project #
3R35GM118026-05S1
Application #
10134562
Study Section
Special Emphasis Panel (ZGM1)
Program Officer
Keane-Myers, Andrea
Project Start
2016-05-01
Project End
2021-04-30
Budget Start
2020-05-01
Budget End
2021-04-30
Support Year
5
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
Columbia University (N.Y.)
Department
Biochemistry
Type
Schools of Medicine
DUNS #
621889815
City
New York
State
NY
Country
United States
Zip Code
10032

  Publications

De Tullio, Luisina; Kaniecki, Kyle; Greene, Eric C (2018) Single-Stranded DNA Curtains for Studying the Srs2 Helicase Using Total Internal Reflection Fluorescence Microscopy. Methods Enzymol 600:407-437
Crickard, J Brooks; Kaniecki, Kyle; Kwon, YoungHo et al. (2018) Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments. J Biol Chem 293:4191-4200
Crickard, J Brooks; Kaniecki, Kyle; Kwon, YoungHo et al. (2018) Regulation of Hed1 and Rad54 binding during maturation of the meiosis-specific presynaptic complex. EMBO J 37:
Crickard, J Brooks; Kaniecki, Kyle; Kwon, Youngho et al. (2018) Meiosis-specific recombinase Dmc1 is a potent inhibitor of the Srs2 antirecombinase. Proc Natl Acad Sci U S A 115:E10041-E10048
Kaniecki, Kyle; De Tullio, Luisina; Greene, Eric C (2018) A change of view: homologous recombination at single-molecule resolution. Nat Rev Genet 19:191-207
Crickard, J Brooks; Greene, Eric C (2018) Biochemical attributes of mitotic and meiotic presynaptic complexes. DNA Repair (Amst) :
Kaniecki, Kyle; De Tullio, Luisina; Gibb, Bryan et al. (2017) Dissociation of Rad51 Presynaptic Complexes and Heteroduplex DNA Joints by Tandem Assemblies of Srs2. Cell Rep 21:3166-3177
Ma, C J; Steinfeld, J B; Greene, E C (2017) Single-Stranded DNA Curtains for Studying Homologous Recombination. Methods Enzymol 582:193-219
De Tullio, Luisina; Kaniecki, Kyle; Kwon, Youngho et al. (2017) Yeast Srs2 Helicase Promotes Redistribution of Single-Stranded DNA-Bound RPA and Rad52 in Homologous Recombination Regulation. Cell Rep 21:570-577
Zhao, Weixing; Steinfeld, Justin B; Liang, Fengshan et al. (2017) BRCA1-BARD1 promotes RAD51-mediated homologous DNA pairing. Nature 550:360-365

Showing the most recent 10 out of 25 publications