Proteins are capable of performing a wide variety of complex molecular functions and play a central role in all biological processes. A detailed and quantitative understanding of the relationship between a protein's sequence and it's biochemical properties would have a profound impact across all areas of biology, medicine, and biotechnology. We are developing data-driven approaches for dissecting the molecular basis of protein function. Our general framework involves designing informative libraries of protein sequences, experimentally mapping the relationship between sequence and function, and extracting detailed functional information from large sequence-function data sets. This work leverages emerging technologies and methods in DNA sequencing and synthesis, microfluidic screening, large-scale statistical learning, and optimization. We will develop generalizable platforms that can be applied to study a wide variety of enzymes and membrane transport proteins.

Public Health Relevance

A detailed and quantitative understanding of the relationship between a protein's sequence and it's biochemical properties would have a profound impact across all areas of biology, medicine, and biotechnology. This important capability would allow us to design new therapeutic and diagnostic proteins, and diagnose genetic diseases before they manifest symptoms. The goal of this proposal is to develop a general framework for dissecting the molecular basis of protein structure, function, and regulation.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Unknown (R35)
Project #
5R35GM119854-05
Application #
9979906
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Krepkiy, Dmitriy
Project Start
2016-08-01
Project End
2021-07-31
Budget Start
2020-08-01
Budget End
2021-07-31
Support Year
5
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Biochemistry
Type
Earth Sciences/Resources
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715