Abstract

Public Health Relevance

Infections caused by herpes simplex viruses remain major health problems in the U.S. The genital form, HSV2 facilitates spread of HIV-AIDS. An effective vaccine against HSV2 is urgently needed. HSV uses 4 proteins, gB, gD and a gH/gL complex to invade cells. We will study how they work together and how the host responds with the long term goal of making effective vaccines and new therapeutics.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI018289-30
Application #
8039969
Study Section
Special Emphasis Panel (NSS)
Program Officer
Challberg, Mark D
Project Start
1981-09-30
Project End
2015-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
30
Fiscal Year
2011
Total Cost
$390,103
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Microbiology/Immun/Virology
Type
Schools of Dentistry
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Cairns, Tina M; Huang, Zhen-Yu; Gallagher, John R et al. (2015) Patient-Specific Neutralizing Antibody Responses to Herpes Simplex Virus Are Attributed to Epitopes on gD, gB, or Both and Can Be Type Specific. J Virol 89:9213-31
Saw, Wan Ting; Matsuda, Zene; Eisenberg, Roselyn J et al. (2015) Using a split luciferase assay (SLA) to measure the kinetics of cell-cell fusion mediated by herpes simplex virus glycoproteins. Methods 90:68-75
Cairns, Tina M; Huang, Zhen-Yu; Whitbeck, J Charles et al. (2014) Dissection of the antibody response against herpes simplex virus glycoproteins in naturally infected humans. J Virol 88:12612-22
Gallagher, John R; Atanasiu, Doina; Saw, Wan Ting et al. (2014) Functional fluorescent protein insertions in herpes simplex virus gB report on gB conformation before and after execution of membrane fusion. PLoS Pathog 10:e1004373
Whitbeck, J Charles; Huang, Zhen-Yu; Cairns, Tina M et al. (2014) Repertoire of epitopes recognized by serum IgG from humans vaccinated with herpes simplex virus 2 glycoprotein D. J Virol 88:7786-95
Cairns, Tina M; Fontana, Juan; Huang, Zhen-Yu et al. (2014) Mechanism of neutralization of herpes simplex virus by antibodies directed at the fusion domain of glycoprotein B. J Virol 88:2677-89
Lazear, Eric; Whitbeck, J Charles; Zuo, Yi et al. (2014) Induction of conformational changes at the N-terminus of herpes simplex virus glycoprotein D upon binding to HVEM and nectin-1. Virology 448:185-95
Krummenacher, Claude; Carfí, Andrea; Eisenberg, Roselyn J et al. (2013) Entry of herpesviruses into cells: the enigma variations. Adv Exp Med Biol 790:178-95
Atanasiu, Doina; Saw, Wan Ting; Gallagher, John R et al. (2013) Dual split protein-based fusion assay reveals that mutations to herpes simplex virus (HSV) glycoprotein gB alter the kinetics of cell-cell fusion induced by HSV entry glycoproteins. J Virol 87:11332-45
Atanasiu, Doina; Cairns, Tina M; Whitbeck, J Charles et al. (2013) Regulation of herpes simplex virus gB-induced cell-cell fusion by mutant forms of gH/gL in the absence of gD and cellular receptors. MBio 4:

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