Abstract

The long-term goal of these studies is to understand the molecular basis of assembly of specific tissue and organ structures by testing the hypothesis that tissue dependent expression of transcript and protein variants determines the specific micro-architecture of the extracellular matrix in different tissues. To reach this goals, we will use a combination of DNA cloning, immunohistochemistry, and transgenic mice technology to analyze the differential expression and interaction properties of the FACIT components IX, XII, and XIV. We will also study the regulated expression and biological role of a novel collagen (type XVII) with multiple triple-helical domains and sequence characteristics typical of proteoglycans. We believe our studies will contribute significantly to the molecular understanding of tissue-dependent diversity of extracellular matrices. This information is essential for understanding normal tissue and organ morphogenesis as well as connective tissue disease processes and genetic abnormalities.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
2R37AR036820-09
Application #
3481584
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1985-07-01
Project End
1998-06-30
Budget Start
1993-07-12
Budget End
1994-06-30
Support Year
9
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Huang, Wei; Li, Qing; Amiry-Moghaddam, Mahmood et al. (2016) Critical Endothelial Regulation by LRP5 during Retinal Vascular Development. PLoS One 11:e0152833
Duan, Xuchen; Murata, Yurie; Liu, Yanqiu et al. (2015) Vegfa regulates perichondrial vascularity and osteoblast differentiation in bone development. Development 142:1984-91
Duncan, Michael B; Yang, Changqing; Tanjore, Harikrishna et al. (2013) Type XVIII collagen is essential for survival during acute liver injury in mice. Dis Model Mech 6:942-51
Liu, Yanqiu; Berendsen, Agnes D; Jia, Shidong et al. (2012) Intracellular VEGF regulates the balance between osteoblast and adipocyte differentiation. J Clin Invest 122:3101-13
Ionescu, Andreia; Kozhemyakina, Elena; Nicolae, Claudia et al. (2012) FoxA family members are crucial regulators of the hypertrophic chondrocyte differentiation program. Dev Cell 22:927-39
Jinnin, Masatoshi; Medici, Damian; Park, Lucy et al. (2008) Suppressed NFAT-dependent VEGFR1 expression and constitutive VEGFR2 signaling in infantile hemangioma. Nat Med 14:1236-46
Saharinen, Pipsa; Eklund, Lauri; Miettinen, Juho et al. (2008) Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts. Nat Cell Biol 10:527-37
Marneros, Alexander G; She, Haicheng; Zambarakji, Hadi et al. (2007) Endogenous endostatin inhibits choroidal neovascularization. FASEB J 21:3809-18
Ueki, Yasuyoshi; Lin, Chin-Yu; Senoo, Makoto et al. (2007) Increased myeloid cell responses to M-CSF and RANKL cause bone loss and inflammation in SH3BP2 ""cherubism"" mice. Cell 128:71-83
Eklund, Lauri; Olsen, Bjorn R (2006) Tie receptors and their angiopoietin ligands are context-dependent regulators of vascular remodeling. Exp Cell Res 312:630-41

Showing the most recent 10 out of 120 publications