Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37CA051605-07
Application #
2094334
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1989-09-30
Project End
1999-11-30
Budget Start
1995-12-01
Budget End
1996-11-30
Support Year
7
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Washington
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
Schultz, Sharon J; Zhang, Miaohua; Champoux, James J (2010) Multiple nucleotide preferences determine cleavage-site recognition by the HIV-1 and M-MuLV RNases H. J Mol Biol 397:161-78
Champoux, James J; Schultz, Sharon J (2009) Ribonuclease H: properties, substrate specificity and roles in retroviral reverse transcription. FEBS J 276:1506-16
Schultz, Sharon J; Zhang, Miaohua; Champoux, James J (2009) Preferred sequences within a defined cleavage window specify DNA 3' end-directed cleavages by retroviral RNases H. J Biol Chem 284:32225-38
Schultz, Sharon J; Zhang, Miaohua; Champoux, James J (2004) Recognition of internal cleavage sites by retroviral RNases H. J Mol Biol 344:635-52
Winshell, Jamie; Paulson, Benjamin A; Buelow, Ben D et al. (2004) Requirements for DNA unpairing during displacement synthesis by HIV-1 reverse transcriptase. J Biol Chem 279:52924-33
Lanciault, Christian; Champoux, James J (2004) Single unpaired nucleotides facilitate HIV-1 reverse transcriptase displacement synthesis through duplex RNA. J Biol Chem 279:32252-61
Winshell, J; Champoux, J J (2001) Structural alterations in the DNA ahead of the primer terminus during displacement synthesis by reverse transcriptases. J Mol Biol 306:931-43
Kelleher, C D; Champoux, J J (2000) RNA degradation and primer selection by Moloney murine leukemia virus reverse transcriptase contribute to the accuracy of plus strand initiation. J Biol Chem 275:13061-70
Schultz, S J; Zhang, M; Kelleher, C D et al. (2000) Analysis of plus-strand primer selection, removal, and reutilization by retroviral reverse transcriptases. J Biol Chem 275:32299-309
Schultz, S J; Zhang, M; Kelleher, C D et al. (1999) Polypurine tract primer generation and utilization by Moloney murine leukemia virus reverse transcriptase. J Biol Chem 274:34547-55

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