Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
4R37DK021491-19
Application #
2137578
Study Section
Special Emphasis Panel (NSS)
Project Start
1978-04-01
Project End
1999-06-30
Budget Start
1996-07-25
Budget End
1997-06-30
Support Year
19
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Medical College of Wisconsin
Department
Biochemistry
Type
Schools of Medicine
DUNS #
073134603
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
Montgomery, Christa; Pei, Zhengtong; Watkins, Paul A et al. (2012) Identification and characterization of an extramitochondrial human 3-hydroxy-3-methylglutaryl-CoA lyase. J Biol Chem 287:33227-36
Miziorko, Henry M (2011) Enzymes of the mevalonate pathway of isoprenoid biosynthesis. Arch Biochem Biophys 505:131-43
Fu, Zhuji; Runquist, Jennifer A; Montgomery, Christa et al. (2010) Functional insights into human HMG-CoA lyase from structures of Acyl-CoA-containing ternary complexes. J Biol Chem 285:26341-9
Fu, Zhuji; Runquist, Jennifer A; Forouhar, Farhad et al. (2006) Crystal structure of human 3-hydroxy-3-methylglutaryl-CoA Lyase: insights into catalysis and the molecular basis for hydroxymethylglutaric aciduria. J Biol Chem 281:7526-32
Theisen, Michael J; Misra, Ila; Saadat, Dana et al. (2004) 3-hydroxy-3-methylglutaryl-CoA synthase intermediate complex observed in ""real-time"". Proc Natl Acad Sci U S A 101:16442-7
Wang, Chang-Zeng; Misra, Ila; Miziorko, Henry M (2004) Utility of acetyldithio-CoA in detecting the influence of active site residues on substrate enolization by 3-hydroxyl-3-methylglutaryl-CoA synthase. J Biol Chem 279:40283-8
Tuinstra, Robbyn L; Wang, Chang-Zeng; Mitchell, Grant A et al. (2004) Evaluation of 3-hydroxy-3-methylglutaryl-coenzyme A lyase arginine-41 as a catalytic residue: use of acetyldithio-coenzyme A to monitor product enolization. Biochemistry 43:5287-95
Misra, Ila; Wang, Chang-Zeng; Miziorko, Henry M (2003) The influence of conserved aromatic residues in 3-hydroxy-3-methylglutaryl-CoA synthase. J Biol Chem 278:26443-9
Tuinstra, Robbyn L; Miziorko, Henry M (2003) Investigation of conserved acidic residues in 3-hydroxy-3-methylglutaryl-CoA lyase: implications for human disease and for functional roles in a family of related proteins. J Biol Chem 278:37092-8
Miziorko, Henry M; Vinarov, Dmitriy A (2002) Detection of covalent tetrahedral adducts by differential isotope shift 13C NMR: acetyl-enzyme reaction intermediate formed by 3-hydroxy-3-methylglutaryl-CoA synthase. Methods Enzymol 354:208-23

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