Abstract

The nuclear matrix of the mammalian nucleus has been isolated and characterized. This cellular structural component appears to be involved in the nuclear functions such as DNA synthesis. The nuclear matrix of the immature rat uterus has high affinity, saturable estrogen binding sites which appears to account for a considerable portion of the salt resistant nuclear estrogen receptors reported by others. The nuclear matrix of the uterus and prostate will be characterized and studied for their binding properties with sex steroid and antisteroid compounds. The nuclear matrix may be associated with a residual structural component extending throughout the cell and is termed the cell matrix. Steroid binding to these components will also be examined.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37DK022000-18
Application #
3483410
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1978-09-01
Project End
1993-08-31
Budget Start
1989-09-01
Budget End
1990-08-31
Support Year
18
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Takaha, Natsuki; Resar, Linda M S; Vindivich, Don et al. (2004) High mobility group protein HMGI(Y) enhances tumor cell growth, invasion, and matrix metalloproteinase-2 expression in prostate cancer cells. Prostate 60:160-7
Takaha, Natsuki; Hawkins, Anita L; Griffin, Constance A et al. (2002) High mobility group protein I(Y): a candidate architectural protein for chromosomal rearrangements in prostate cancer cells. Cancer Res 62:647-51
Lupold, Shawn E; Hicke, Brian J; Lin, Yun et al. (2002) Identification and characterization of nuclease-stabilized RNA molecules that bind human prostate cancer cells via the prostate-specific membrane antigen. Cancer Res 62:4029-33
De Marzo, A M; Nelson, W G; Meeker, A K et al. (1998) Stem cell features of benign and malignant prostate epithelial cells. J Urol 160:2381-92
De Marzo, A M; Meeker, A K; Epstein, J I et al. (1998) Prostate stem cell compartments: expression of the cell cycle inhibitor p27Kip1 in normal, hyperplastic, and neoplastic cells. Am J Pathol 153:911-9
Nardozza, T A; Quigley, M M; Getzenberg, R H (1996) Association of transcription factors with the nuclear matrix. J Cell Biochem 61:467-77
Luke, M C; Coffey, D S (1994) Human androgen receptor binding to the androgen response element of prostate specific antigen. J Androl 15:41-51
Murphy, B C; Pienta, K J; Coffey, D S (1992) Effects of extracellular matrix components and dihydrotestosterone on the structure and function of human prostate cancer cells. Prostate 20:29-41
Partin, A W; Steinberg, G D; Pitcock, R V et al. (1992) Use of nuclear morphometry, gleason histologic scoring, clinical stage, and age to predict disease-free survival among patients with prostate cancer. Cancer 70:161-8
Pienta, K J; Getzenberg, R H; Coffey, D S (1992) Characterization of nuclear morphology and nuclear matrices in ageing human fibroblasts. Mech Ageing Dev 62:13-24

Showing the most recent 10 out of 23 publications