The proposed research focuses on the aqueous humor circulatory system of the human eye. Methods will be developed and employed to measure the rate of circulation of aqueous humor in the human eye under a variety of circumstances in order to determine its physiological regulation, its circadian rhythms, its response to disease, and its responsiveness to ocular hypotensive drugs. The techniques for study are non-invasive and safe, depending on the measurement of steady-state fluorescence of exogenous fluoroprobes, as well as other properties including polarization of fluorescence, spectra of fluorescence, and excited state lifetime of fluorescence. Studies of normal eyes and a number of clinical disorders including cystic fibrosis, diabetes insipidus, glaucoma, diabetes mellitus, central retinal vein occlusion, and uveitis will be made to characterize the characteristics and responsiveness of the aqueous circulation in these disorders. Studies of a number of pharmacological agents will be conducted including those whose effects are mediated via cyclic nucleotides, calcium channels, angiotensin, and polyphosphoinositides to gain general principles about the pharmacologic responsiveness of this circulatory system.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37EY000634-28
Application #
2608546
Study Section
Special Emphasis Panel (NSS)
Project Start
1976-03-01
Project End
1999-11-30
Budget Start
1997-12-01
Budget End
1998-11-30
Support Year
28
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
City
Rochester
State
MN
Country
United States
Zip Code
55905
McLaren, Jay W; Herman, David C; Brubaker, Richard F et al. (2003) Effect of ibopamine on aqueous humor production in normotensive humans. Invest Ophthalmol Vis Sci 44:4853-8
Leavitt, Jacqueline A; Wayman, Laura L; Hodge, David O et al. (2002) Pupillary response to four concentrations of pilocarpine in normal subjects: application to testing for Adie tonic pupil. Am J Ophthalmol 133:333-6
Brubaker, R F; Schoff, E O; Nau, C B et al. (2001) Effects of AGN 192024, a new ocular hypotensive agent, on aqueous dynamics. Am J Ophthalmol 131:19-24
Brubaker, R F; Ingram, C J; Schoff, E O et al. (2000) Comparison of the efficacy of betaxolol-brinzolamide and timolol-dorzolamide as suppressors of aqueous humor flow in human subjects. Ophthalmology 107:283-7
Brubaker, R F; Bourne, W M; Bachman, L A et al. (2000) Ascorbic acid content of human corneal epithelium. Invest Ophthalmol Vis Sci 41:1681-3
Maus, T L; Brubaker, R F (1999) Measurement of aqueous humor flow by fluorophotometry in the presence of a dilated pupil. Invest Ophthalmol Vis Sci 40:542-6
McLaren, J W; Bachman, L A; Brubaker, R F (1999) Comparison of effects of topical ibopamine and epinephrine on the circadian rhythm of intraocular pressure of the rabbit eye as measured by telemetry. J Ocul Pharmacol Ther 15:107-16
Ingram, C J; Brubaker, R F (1999) Effect of brinzolamide and dorzolamide on aqueous humor flow in human eyes. Am J Ophthalmol 128:292-6
Maus, T L; Nau, C; Brubaker, R F (1999) Comparison of the early effects of brimonidine and apraclonidine as topical ocular hypotensive agents. Arch Ophthalmol 117:586-91
Schadlu, R; Maus, T L; Nau, C B et al. (1998) Comparison of the efficacy of apraclonidine and brimonidine as aqueous suppressants in humans. Arch Ophthalmol 116:1441-4

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