Abstract

Detailed information is available regarding the mechanisms of regulating specific genes in bacteria. Much less is known about how these individual control systems interact so as to coordinate the functions of multigene systems. We propose to genetically approach control of several multigene systems and how they might interact. We will work on 1) the histidine operon and how it interacts with the related purine pathway and the cells control of cell division, 2) the purine pathway per se will be investigated to determine how the many genes in its separate branches are controlled, 3) a new system of gene regulation will be investigated which we believe is related to purine metabolism and involves structural alteration of DNA. The system seems to cause reversible inactivation of cryptic prophages, 4) the NAD pathway involves not only biosynthesis but also recycling pathway for salvage of pyridine nucleotides. This system is involved in DNA repair as well as oxidation/reduction reactions. The control of this complex pathway and its integration with cellular regulatory mechanisms will be pursued. All of these endeavors will involve a heavily genetic approach employing transposable elements, some of which form operon fusions of the lac operon to the promoter of the target gene. Many of the methods used are one developed in this lab.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37GM023408-14
Application #
3484470
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1977-01-01
Project End
1991-12-31
Budget Start
1990-01-01
Budget End
1990-12-31
Support Year
14
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of Utah
Department
Type
Schools of Arts and Sciences
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Li, Chan; Edwards, Michelle D; Jeong, Hotcherl et al. (2007) Identification of mutations that alter the gating of the Escherichia coli mechanosensitive channel protein, MscK. Mol Microbiol 64:560-74
Grose, Julianne H; Joss, Lisa; Velick, Sidney F et al. (2006) Evidence that feedback inhibition of NAD kinase controls responses to oxidative stress. Proc Natl Acad Sci U S A 103:7601-6
Cornish, Rita M; Roth, John R; Poulter, C Dale (2006) Lethal mutations in the isoprenoid pathway of Salmonella enterica. J Bacteriol 188:1444-50
Grose, Julianne H; Bergthorsson, Ulfar; Roth, John R (2005) Regulation of NAD synthesis by the trifunctional NadR protein of Salmonella enterica. J Bacteriol 187:2774-82
Grose, Julianne H; Bergthorsson, Ulfar; Xu, Yaping et al. (2005) Assimilation of nicotinamide mononucleotide requires periplasmic AphA phosphatase in Salmonella enterica. J Bacteriol 187:4521-30
Xu, K; Delling, J; Elliott, T (1992) The genes required for heme synthesis in Salmonella typhimurium include those encoding alternative functions for aerobic and anaerobic coproporphyrinogen oxidation. J Bacteriol 174:3953-63
Downs, D M; Roth, J R (1991) Synthesis of thiamine in Salmonella typhimurium independent of the purF function. J Bacteriol 173:6597-604
Hughes, K T; Roth, J R; Olivera, B M (1991) A genetic characterization of the nadC gene of Salmonella typhimurium. Genetics 127:657-70
Zhu, N; Olivera, B M; Roth, J R (1989) Genetic characterization of the pnuC gene, which encodes a component of the nicotinamide mononucleotide transport system in Salmonella typhimurium. J Bacteriol 171:4402-9
Zhu, N; Olivera, B M; Roth, J R (1988) Identification of a repressor gene involved in the regulation of NAD de novo biosynthesis in Salmonella typhimurium. J Bacteriol 170:117-25

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