The cell cycle defines an orderly series of events that together make up the process of cell duplication. Research over the past thirty years has confirmed that most of the fundamental cell cycle events are conserved across all eukaryotic lineages. We have undertaken a genetic analysis of cell cycle events in yeast under the assumption that findings made using this simple genetically tractable organism will be of general interest and utility. The experiments proposed are aimed at elucidating the molecular basis for cell cycle progression and control in three general areas: (l) regulation of the cell cycle by proteolysis of key regulatory proteins; (2) regulation of mitotic spindle development; and (3) cell cycle regulation of cellular morphogenesis. In each case, a strategy of combining molecular and genetic approaches, that has proved extremely successful with yeast, is planned. It is anticipated that the proposed research will synergize with work in other experimental systems, and thus contribute to the general elucidation of the molecular basis for cell cycle progression and control. Ultimately, such information will be important for the diagnosis and treatment of human diseases such as cancer.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37GM038328-20
Application #
7015611
Study Section
Special Emphasis Panel (NSS)
Program Officer
Zatz, Marion M
Project Start
1987-03-01
Project End
2010-02-28
Budget Start
2006-03-01
Budget End
2007-02-28
Support Year
20
Fiscal Year
2006
Total Cost
$446,082
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037
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Baskerville, Chris; Segal, Marisa; Reed, Steven I (2008) The protease activity of yeast separase (esp1) is required for anaphase spindle elongation independently of its role in cleavage of cohesin. Genetics 178:2361-72
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Tang, Carol S L; Reed, Steven I (2002) Phosphorylation of the septin cdc3 in g1 by the cdc28 kinase is essential for efficient septin ring disassembly. Cell Cycle 1:42-9
Segal, Marisa; Bloom, Kerry; Reed, Steven I (2002) Kar9p-independent microtubule capture at Bud6p cortical sites primes spindle polarity before bud emergence in Saccharomyces cerevisiae. Mol Biol Cell 13:4141-55
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Bertolaet, B L; Clarke, D J; Wolff, M et al. (2001) UBA domains mediate protein-protein interactions between two DNA damage-inducible proteins. J Mol Biol 313:955-63
Haase, S B; Winey, M; Reed, S I (2001) Multi-step control of spindle pole body duplication by cyclin-dependent kinase. Nat Cell Biol 3:38-42

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