Sertoli cells are the epithelial """"""""nurse"""""""" cells of the testis which along with the germ cells comprise the seminiferous tubules. The Sertoli cells clearly play important although incompletely defined roles in the process of spermatogenesis. The long term goals of this project are to understand the different roles of the Sertoli cells in spermatogenesis. These goals relate directly to human health problems dealing with reproductive infertility and may lead to new approaches to contraception. In the past grant periods this project has focused on the characterization of Sertoli cell secretion products and their specific roles in spermatogenesis. In the next grant period we propose logical and focused extensions of our previous studies, and, in addition, propose some new approaches to understanding how Sertoli cells products influence germ cells.
The specific aims for the next grant period include: 1. The identification and characterization of regulatory elements on the FSH receptor (FSHR) gene promoter. 2. Identification of agents which regulate the expression of the FSH receptor in Sertoli cells in culture and in vivo. 3. Successful direction of transgenes into the Sertoli cells of transgenic mice. We hope that this last aim will lead to ways to specifically alter the expression of genes in Sertoli cells in vivo.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
2R37HD010808-20
Application #
2461527
Study Section
Reproductive Biology Study Section (REB)
Project Start
1977-08-01
Project End
2002-03-31
Budget Start
1997-04-01
Budget End
1998-03-31
Support Year
20
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Washington State University
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
041485301
City
Pullman
State
WA
Country
United States
Zip Code
99164
Griswold, Michael D; Hogarth, Cathryn (2018) Beyond stem cells: Commitment of progenitor cells to meiosis. Stem Cell Res 27:169-171
Griswold, Michael D (2016) Spermatogenesis: The Commitment to Meiosis. Physiol Rev 96:1-17
Chen, Yao; Ma, Li; Hogarth, Cathryn et al. (2016) Retinoid signaling controls spermatogonial differentiation by regulating expression of replication-dependent core histone genes. Development 143:1502-11
Hogarth, Cathryn A; Evans, Elizabeth; Onken, Jennifer et al. (2015) CYP26 Enzymes Are Necessary Within the Postnatal Seminiferous Epithelium for Normal Murine Spermatogenesis. Biol Reprod 93:19
Evans, Elizabeth; Hogarth, Cathryn; Mitchell, Debra et al. (2014) Riding the spermatogenic wave: profiling gene expression within neonatal germ and sertoli cells during a synchronized initial wave of spermatogenesis in mice. Biol Reprod 90:108
Hogarth, Cathryn A; Griswold, Michael D (2013) Retinoic acid regulation of male meiosis. Curr Opin Endocrinol Diabetes Obes 20:217-23
Hogarth, Cathryn A; Evanoff, Ryan; Mitchell, Debra et al. (2013) Turning a spermatogenic wave into a tsunami: synchronizing murine spermatogenesis using WIN 18,446. Biol Reprod 88:40
Tong, Ming-Han; Yang, Qi-En; Davis, Jeffrey C et al. (2013) Retinol dehydrogenase 10 is indispensible for spermatogenesis in juvenile males. Proc Natl Acad Sci U S A 110:543-8
Evans, Elizabeth B; Hogarth, Cathryn; Evanoff, Ryan M et al. (2012) Localization and regulation of murine Esco2 during male and female meiosis. Biol Reprod 87:61
Ray, Debjit; Hogarth, Cathryn A; Evans, Elizabeth B et al. (2012) Experimental validation of Ankrd17 and Anapc10, two novel meiotic genes predicted by computational models in mice. Biol Reprod 86:102

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